Fig. 2: Reversible changes in mitochondrial morphology and function. | Cell Death Discovery

Fig. 2: Reversible changes in mitochondrial morphology and function.

From: PGC-1a mediated mitochondrial biogenesis promotes recovery and survival of neuronal cells from cellular degeneration

Fig. 2

A Original fluorescence and 3D reconstruction images of Mito-tracker stained mitochondria in neuronal PC12 cells showed mitochondrial fragmentation induced by exposure to EtOH (5%, vol/vol, 1 and 3 h) and subsequent recovery of mitochondrial morphology after washing EtOH (Washed, 4 and 20 h). Scale bar: 20 µm. B Quantification result of the percentage of cells with mitochondrial fragmentation. Data are presented as mean ± SEM. ****P < 0.0001, vs. control group; ####P < 0.0001, vs. EtOH 3 h group. C–E Quantification results of mitochondrial number, mean mitochondrial volume and total mitochondrial volume per cell. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001, vs. control group; ##P < 0.01, ####P < 0.0001, vs. EtOH 3 h group. F Quantification of mean fluorescence intensity stained with TMRM. Data are presented as mean ± SEM. ***P < 0.001, vs. control group; ##P < 0.01, ###P < 0.001, vs. EtOH 3 h group. G ATP content (nmol/mg protein) in neuronal PC12 cells. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001, vs. control group; ##P < 0.01, ###P < 0.001, vs. EtOH 3 h group. H The relative mtDNA copy number estimated with qPCR measurement. Data are presented as mean ± SEM. EtOH ethanol.

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