Fig. 5: PCK1 overexpression strengthens proliferation and migration of uveal melanoma cells.

The stable 92.1 cells with the lentivirus-packed PCK1-expressing construct (“oePCK1”) or the empty vector (“Vec”) were established and expression of PCK1/2 was measured (A and B). An equal number of the aforementioned 92.1 cells were cultured for specified durations, and cell viability, proliferation, in vitro cell migration and invasion were tested via CCK-8 (C), EdU-nuclei staining (D), “Transwell” (E) and “Matrigel Transwell” (F) assays, respectively. The patient-derived primary human UVM cells, priUVM, and the immortalized lines (OCM-1 and Mel202) were engineered to stably express either the lentivirus-packed PCK1-expressing construct (“oePCK1”) or the empty vector (“Vec”), and PCK1/2 mRNA expression was tested (G, H). An equal number of the aforementioned UVM cells were cultured for specified durations, and proliferation and in vitro cell migration were tested via measuring EdU-nuclei ratio (I) and “Transwell” (J) assays, respectively, with results quantified. The numerical values are presented as the mean ± standard deviation (SD). “Pare” signifies the parental control cells. *Indicates statistical significance (P < 0.05) when compared to “Vec” cells. “N. S.” denotes a lack of statistical difference (P > 0.05). The experiments depicted in this figure were replicated five times (n = 5, biological repeats), consistently yielding similar results. The scale bar corresponds to 100 μm.