Fig. 2: H101 activates cGAS expression and GAU1 lncRNA inhibits over-expression of NF-κB p65.

A Real-time PCR analysis revealed the expression of cGAS mRNA levels following H101 infection in SW620 and LOVO cells. All experiments were conducted 72 hours after H101 infection (MOI = 100). GAPDH was utilized as the internal control for normalization. The data are presented as the mean ± SD, and the differences between the two groups were calculated using an unpaired two-tailed t-test. Statistical significance was denoted as **P < 0.01 and *P < 0.05, N = 3. B Western blot analysis showed the level of cGAS protein after 72 hours of H101 infection in SW620 and LOVO cells. C Real-time PCR showed that the GAU1 lncRNA expression in tumor cells. Data are presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, **P < 0.01 compared with NCM460. D Real-time PCR analysis showed the expression of GAU1 knockdown by CRISPR/dCas9-KRBA method in SW620 and LOVO cells. Data are presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, ****P < 0.0001 compared with empty vector (Empty Vector, EV: cells transfected with the empty dCas9-KRAB vector). E Real-time PCR showed the NF-κB p65 mRNA levels in SW620 and LOVO tumor cells after GAU1 knockdown. GAPDH was used as the internal control for normalization. Data are presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, **P < 0.01. F Western blot confirmed the expression of NF-κB p65 protein after GAU1 knockdown in SW620 and LOVO tumor cells.