Fig. 3: The IDTR strategy inhibits tumor growth in colorectal tumor cells.
From: Induced dual-target rebalance simultaneously enhances efficient therapeutical efficacy in tumors
A Cell growth rate was measured by CCK8 assay on days 0,1, 2, 3, 4 and 5 after the cells were treated with EV (Square), H101(Cycle), GAU1-sgRNA (Triangle) and H101 + GAU1-sgRNA (Inverted triangle) in the LOVO cells. B Cell growth rate was measured by CCK8 assay in the SW620 cells. Experimental methods and annotations were described above. C Cell growth rate was measured by CCK8 assay in the NCM460 normal cells. Experimental methods and annotations were described above. D Cloning formation assay was conducted to assess the colony formation ability of EV, EV + H101, GAU1-sgRNA, H101 + GAU1-sgRNA. E Quantification of visible colonies was performed. The colony count in the empty vector group was set as 100%. All experiments were performed in triplicate, and the relative colony formation rates were showed as the mean ± SEM. N = 3, ****P < 0.0001. F Representative micrographs from LOVO cells treated with 10 μM EdU for 4 hours were provided. Each type of cell was categorized based on treatment: EV (Lane 1), H101(Lane 2), GAU1-sgRNA (Lane 3) and H101 + GAU1-sgRNA (Lane 4) groups. Blue: Hoechst 33342 staining of nuclear; RED: Alexa Fluor 555 staining of proliferating cells. The scale bar was 5 mm. G LOVO cells proliferation quantification. The proportion of proliferating cells in the empty vector group was set to 1. The proportion of proliferating cells in the other experimental groups was compared to that in the empty vector group. Data were presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, ****P < 0.0001. H Representative micrographs from SW620 cells treated with 10 μM EdU for 4 hours were provided. Experimental methods and groupings were described above. The scale bar was 5 mm. I SW620 cells proliferation quantification. Experimental methods, grouping and data processing were described above. Data were presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, ****P < 0.0001 and ***P < 0.001.
