Fig. 5: There is a lack of cross-talk between GAU1 knockdown and H101 infection via the IDTR strategy.
From: Induced dual-target rebalance simultaneously enhances efficient therapeutical efficacy in tumors
A Western blot showed the expression of cGAS protein in SW620 cells. The experiments were categorized into empty vector (EV): cells transfected with the empty dCas9-KRAB vector (Lane 1), EV with H101 infection (Multiplicity of Infection (MOI) = 100) after 72 hours (EV + H101) (Lane 2), stable transformation in GAU1 knockdown (GAU1 sgRNA) (Lane 3), H101 + GAU1 sgRNA (Lane 4). B Western blot showed the expression of cGAS protein in LOVO cells. C Western blot data the expression of cGAS protein was automatically analyzed by ImageJ. GAPDH was used as the internal control for normalization. Data were presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, ****P < 0.0001, ***P < 0.001 and **P < 0.01. D Western blot showed the expression of NF-κB p65 protein in SW620 cells. Treatment methods and control are the same as above. E Western blot showed the expression of NF-κB p65 protein in LOVO cells. F Western blot data of the expression of NF-κB p65 protein was automatically analyzed by ImageJ. Data were presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed t-test. N = 3, ****P < 0.0001 and ***P < 0.001. Western blot data was automatically analyzed by ImageJ.
