Fig. 1: Triple knockout of Rsk1, Rsk2, and Rsk3 enhances TNF-induced mouse death by promoting apoptosis and necroptosis in duodenal epithelial cells. | Cell Death Discovery

Fig. 1: Triple knockout of Rsk1, Rsk2, and Rsk3 enhances TNF-induced mouse death by promoting apoptosis and necroptosis in duodenal epithelial cells.

From: Phosphorylation of caspase-8 by RSKs via organ-constrained effects controls the sensitivity to TNF-induced death

Fig. 1

Rsk1+/+Rsk2+/+Rsk3+/+ and Rsk1−/−Rsk2−/−Rsk3−/− mice were injected intravenously (i.v.) with TNF (0.4 μg/g, n = 6), survival curve (A) and body temperature (B) were recorded at the indicated time. Mouse survival was presented as a Kaplan–Meier plot, and the log-rank test was performed. ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. C Representative images of the gastrointestinal tract from Rsk1+/+Rsk2+/+Rsk3+/+ or Rsk1−/−Rsk2−/−Rsk3−/− mice i.v. injected with 0.4 μg/g TNF for 8 h. D Representative H&E staining images of pathological changes of cecum and duodenum of Rsk1+/+Rsk2+/+Rsk3+/+ and Rsk1−/−Rsk2−/−Rsk3−/− mice i.v. injected with TNF (0.4 μg/g) for 8 h. Scale bars as indicated. E Representative pMLKL immunochemistry staining images of cecum from Rsk1+/+Rsk2+/+Rsk3+/+ and Rsk1−/−Rsk2−/−Rsk3−/− mice i.v. injected with TNF (0.4 μg/g) for 8 h. Scale bars as indicated. F Representative pMLKL, cleaved Casp3 and cleaved Casp8 immunochemistry staining images of duodenum from Rsk1+/+Rsk2+/+Rsk3+/+ and Rsk1−/−Rsk2−/−Rsk3−/− mice i.v. injected with TNF (0.4 μg/g) for 8 h. Scale bars as indicated.

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