Fig. 2: The T265A mutation of caspase-8 enhances TNF-induced mouse death by promoting apoptosis and necroptosis in duodenal epithelial cells. | Cell Death Discovery

Fig. 2: The T265A mutation of caspase-8 enhances TNF-induced mouse death by promoting apoptosis and necroptosis in duodenal epithelial cells.

From: Phosphorylation of caspase-8 by RSKs via organ-constrained effects controls the sensitivity to TNF-induced death

Fig. 2

Casp8+/+ and Casp8T265A/T265A mice were i.v. injected with TNF (0.4 μg/g, n = 6), survival curve (A), and body temperature (B) were recorded at the indicated time. Mouse survival was presented as a Kaplan–Meier plot, and the log-rank test was performed. ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. C Representative H&E staining images of cecum and duodenum from Casp8+/+ and Caspase-8T265A/T265A mice i.v. injected with TNF (0.4 μg/g) for 8 h. Scale bars as indicated. D Representative images of immunochemistry staining of pMLKL, cleaved Casp3, and cleaved Casp8 in the duodenum of Casp8+/+ and Casp8T265A/T265A mice i.v. injected with TNF (0.4 μg/g) for 8 h. Scale bars as indicated.

Back to article page