Fig. 5: TNF or TNF + zVAD induces more cytokines production in Rsk1−/−Rsk2−/− Rsk3−/− or Casp8T265A/T265A mice than that in WT mice. | Cell Death Discovery

Fig. 5: TNF or TNF + zVAD induces more cytokines production in Rsk1−/−Rsk2−/− Rsk3−/− or Casp8T265A/T265A mice than that in WT mice.

From: Phosphorylation of caspase-8 by RSKs via organ-constrained effects controls the sensitivity to TNF-induced death

Fig. 5

A–D Rsk1+/+Rsk2+/+Rsk3+/+ and Rsk1−/−Rsk2−/−Rsk3−/− mice were i.v. injected with TNF (0.4 μg/g). At the indicated time points, mouse serum was collected for ELISA analysis of IL-1β (A), IL-12/IL-23 (B), IL-6 (C), and IFN-γ (D) (n = 6 per group at each time point). E–H Casp8+/+ and Casp8 T265A/T265A mice were i.v. injected with TNF (0.4 μg/g). At the indicated time points, mouse serum was collected for ELISA analysis of IL-1β (E), IL-12/IL-23 (F), IL-6 (G), and IFN-γ (H) (n = 4 per group at each time point). Mice of indicated genotypes were i.v. injected with TNF (0.4 μg/g). 4 h after TNF injection, mouse serum was collected for ELISA analysis of IL-1β (I), IL-6 (J), and IFN-γ (K) (n = 4 per group at each time point). Mice of indicated genotypes were i.v. injected with TNF (0.4 μg/g) plus i.p injections of zVAD at 15 min before (250 μg per mouse) and 1 h after (100 μg per mouse) TNF injection. 4 h after TNF injection, mouse serum was collected for ELISA analyzation of IL-1β (L), IL-6 (M), and IFN-γ (N) (n = 4 per group at each time point). ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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