Fig. 4: NUP43 enhances the growth and infiltration of CRC cells.

A, B Three shRNAs (sh-NUP43-1#, sh-NUP43-2#, and sh-NUP43-3#) were designed to silence CRC cells (HCT116 and SW480) and verified by qRT-PCR and Western blotting. We used sh-NUP43-3# to downregulate NUP43. NUP43 overexpression was verified in CRC cells (HCT116 and SW480) by qRT-PCR and Western blotting. C The growth curve of cells transfected with sh-NUP43/NUP43 was drawn according to the CCK-8 method. The upper figure shows the cell proliferation results after NUP43 knockdown, and the lower figure shows the cell proliferation results after NUP43 overexpression. D The EdU assay is used to assess the proliferation capacity of CRC cells that have been transfected with sh-NUP43/NUP43. The left panel depicts cellular proliferation following the manipulation of NUP43 through knockdown or overexpression. The findings of the cell counting analysis are displayed in the right section. E Transwell test was used to assess the invasive and metastatic properties of CRC cells that were transfected with sh-NUP43/NUP43. The upper panel displays the outcomes of cell invasion and metastasis following the manipulation of NUP43 through knockdown or overexpression. The lower panel displays the findings of migration and metastatic cell count assessments. F A scratch experiment was conducted to assess the invasive capacity of CRC cells that were transfected with sh-NUP43/NUP43. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.