Fig. 6: IPO5 plays a role in the NUP43-facilitated movement of PD-L1 into the nucleus. | Cell Death Discovery

Fig. 6: IPO5 plays a role in the NUP43-facilitated movement of PD-L1 into the nucleus.

From: NUP43 promotes PD-L1/nPD-L1/PD-L1 feedback loop via TM4SF1/JAK/STAT3 pathway in colorectal cancer progression and metastatsis

Fig. 6

A RNA sequencing was employed to assess the differential expression of mRNAs in the sh-NUP43 and sh-NC groups. In the Volcano plot, the color red represents a higher gene expression level in the sh-NC group compared to the sh-NUP43 group, while the color blue represents a lower expression level. An arrow denotes IPO5. B Heatmap illustrating the expression levels of mRNAs that are differently expressed between the sh-NUP43 and sh-NC groups. The intensity of the color red directly correlates with the degree of expression. C Performing GO analysis on the mRNA in CRC cells treated with sh-NUP43 and sh-NC. D Performing KEGG pathway analysis on mRNA in CRC cells treated with sh-NUP43 and sh-NC. E The expression of IPO5 mRNA was detected by qRT-PCR in cells from each group, namely sh-NUP43, sh-NC, Vector, and NUP43. F, G Performing Western blot analysis to detect the presence of NUP43 and IPO5 proteins in each cell group. H The HEK293T cells were transfected with the specified plasmids. The interaction between PD-L1 and IPO5 was assessed using Western blotting and co-IP techniques. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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