Fig. 7: IPO5 is an indispensable key molecule during the NUP43-mediated nuclear translocation of PD-L1. | Cell Death Discovery

Fig. 7: IPO5 is an indispensable key molecule during the NUP43-mediated nuclear translocation of PD-L1.

From: NUP43 promotes PD-L1/nPD-L1/PD-L1 feedback loop via TM4SF1/JAK/STAT3 pathway in colorectal cancer progression and metastatsis

Fig. 7

A, B Three shRNAs (sh-IPO5-1#, sh-IPO5-2#, and sh-IPO5-3#) were designed to silence CRC cells (HCT116 and SW480) and verified by qRT-PCR and Western blotting. We used sh-IPO5-2# to downregulate IPO5. IPO5 overexpression was verified in CRC cells (HCT116 and SW480) by qRT-PCR and Western blotting. C, D Following the division of cells into several groups (sh-IPO5, sh-NC, Vector, IPO5) based on nucleocytoplasmic distribution, PD-L1 Western blot analysis was conducted. E, F Confocal microscopy was used to assess the immunofluorescence staining of PD-L1 in cells from each group. G qRT-PCR was conducted to identify the presence of NUP43 and IPO5 mRNA in cells from each experimental group (Vector, NUP43, NUP43+sh-NC, and NUP43+sh-IPO5). H, I Perform Western blot analysis to examine the expression levels of IPO5 and PD-L1 in each cell group. J, K Confocal microscopy was used to assess the immunofluorescence staining of PD-L1 in cells from each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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