Fig. 9: TM4SF1 enhances the process of PD-L1 gene transcription by stimulating the JAK/STAT3 signaling pathway. | Cell Death Discovery

Fig. 9: TM4SF1 enhances the process of PD-L1 gene transcription by stimulating the JAK/STAT3 signaling pathway.

From: NUP43 promotes PD-L1/nPD-L1/PD-L1 feedback loop via TM4SF1/JAK/STAT3 pathway in colorectal cancer progression and metastatsis

Fig. 9

A, B, D, E Three shRNAs (sh-TM4SF1-1#, sh-TM4SF1-2#, and sh-TM4SF1-3#) were designed to silence CRC cells (HCT116 and SW480) and were analyzed by qRT-PCR and Western blotting. We used sh-TM4SF1-1# to downregulate TM4SF1. TM4SF1 overexpression was verified in CRC cells (HCT116 and SW480) by qRT-PCR and Western blotting. C, F The Western blot analysis reveals the levels of JAK2, phospho-JAK2, STAT3, and phospho-STAT3 expression in CRC cells with either reduced or increased TM4SF1 expression. G According to the JASPAR database, it was anticipated that the promoter of the PD-L1 gene contains the binding site for STAT3. H The ChIP data for GSM935457, GSM935399, GSM935551, and GSM1227206 revealed a distinct peak of STAT3 located upstream of PD-L1. I Three shRNAs (sh-STAT3-1#, sh-STAT3-2#, and sh-STAT3-3#) were designed to silence CRC cells (HCT116 and SW480) and verified by qRT-PCR. We used sh-STAT3-2# to downregulate STAT3. J The expression of PD-L1 mRNA in cells of each group (sh-NC, sh-STAT3) was detected by qRT-PCR. K Western blot analysis was conducted to assess the efficacy of sh-STAT3 silencing in each group, and to analyze the expression of PD-L1 in cells from each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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