Fig. 3: ML-SI1 promotes ferroptosis in CSCs.
From: Inhibition of lysosomal TRPML1 channel eliminates breast cancer stem cells by triggering ferroptosis
A ROS levels detected using the CM-H2DCFDA dye by flow cytometry in HCC1954 mammosphere cells incubated with DMSO or 10 μM ML-SI1 for 48 h. B Statistics of CM-H2DCFDA signals presented in A. C Confocal images showing lipid ROS visualized by C11-BODIPY in cells isolated from HCC1954 mammospheres and treated with 10 μM ML-SI1 for 48 h. Scale bar = 10 μm. D Statistics of C11-BODIPY signals presented in C. E Immunoblots of the Ferritin levels in HCC1954 mammospheres after 48 h treatment of DMSO or 10 μM ML-SI1. F Quantification of the relative Ferritin levels normalized to ACTB in E. G Images of HCC1954 mammospheres after treatment of 10 μM ML-SI1 alone or 10 μM ML-SI1 + 1 μM Liproxstatin-1 (Lip-1) for 7 days. Scale bar = 200 μm. H The diameters of the mammospheres in G. The relative MDA (I) and GSH (J) contents normalized to that of the control vehicle group in HCC1954 cells treated with 10 μM ML-SI1, 10 μM ML-SI1 + 1 μM Lip-1 or 10 μM ML-SI1 + 20 μM DFO as indicated. K Co-localization of FeRhoNox-1 with Lysotracker Green in HCC1954 cells pre-incubated with DMSO or 10 μM ML-SI1 for 48 h. Scale bar = 10 μm. Data are presented as the means ± SEM.
