Fig. 3: SERCA-mediated intracellular calcium overload contributes to endoplasmic reticulum stress and apoptosis following heat stress. | Cell Death Discovery

Fig. 3: SERCA-mediated intracellular calcium overload contributes to endoplasmic reticulum stress and apoptosis following heat stress.

From: Heat stress induces calcium dyshomeostasis to subsequent cognitive impairment through ERS-mediated apoptosis via SERCA/PERK/eIF2α pathway

Fig. 3: SERCA-mediated intracellular calcium overload contributes to endoplasmic reticulum stress and apoptosis following heat stress.

A Laser scanning confocal microscope observation and quantification of calcium ions in hippocampus of heat-stressed mice. Scale bar “—” =100 μm. B KEGG pathway enrichment analysis for differentially expressed gene. C The effect of heat stress on neuronal endoplasmic reticulum was observed under transmission electron microscope; Arrows indicated morphological swelling changes of endoplasmic reticulum. Scale bar = 500 nm. D Representative western blot bands of P-PERK, PERK, P-eIF2α, eIF2α, CHOP and Caspase-3 in hippocampal tissues. E Immunofluorescence staining was used to determine the alterations in Caspase-3. Scale bar “--” =20 μm. Values are expressed as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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