Fig. 1: Growth factor stimuli or carotid artery ligation upregulates USP14. | Cell Death Discovery

Fig. 1: Growth factor stimuli or carotid artery ligation upregulates USP14.

From: USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein

Fig. 1: Growth factor stimuli or carotid artery ligation upregulates USP14.The alternative text for this image may have been generated using AI.

A, B Cells were starved with serum-free medium for 24 h. Protein lysate was obtained from HASMCs treated with PDGF-BB (10 ng/mL), followed by Western blot. GAPDH was a loading control. C Total RNA was extracted from HASMCs exposed to PDGF-BB. RT-qPCR was applied to evaluate the mRNA level of USP14 (n = 3). D Immunofluorescence staining with USP14 (red), α-SMA (green), and DAPI (blue) on the carotid arteries of ligation and sham groups. E Immunofluorescence staining with USP14 in PDGF-BB-treated HASMCs for 12 h. F Fluorescence intensity was measured using Image J (n = 3). *P < 0.05.

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