Fig. 3: Inhibition or knockdown of USP14 suppresses cell cycle transition and migration. | Cell Death Discovery

Fig. 3: Inhibition or knockdown of USP14 suppresses cell cycle transition and migration.

From: USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein

Fig. 3: Inhibition or knockdown of USP14 suppresses cell cycle transition and migration.The alternative text for this image may have been generated using AI.

A Cell distribution was assessed on VSMCs treated with PDGF-BB or/and IU1 (75 μM) using flow cytometry. C HASMCs were treated with PDGF-BB or/and USP14 siRNA for 48 h, followed by flow cytometry. B, D Cell number was counted at different phases. E Cells were starved for 24 h and exposed to IU1 and/or PDGF-BB for 48 h. Western blot was used to evaluate the expression of Cyclin D1. F HASMCs were treated with USP14 siRNA and/or PDGF-BB for 48 h, followed by Western blot. G A7r5 cells were exposed to PDGF-BB and/or IU1, followed by scratch assay. Images were captured at 0 h and 72 h. H The treated cells were transferred to the transwell for migration. I Protein extracts were collected for Western blot to evaluate MMP2 expression.

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