Fig. 4: USP14 mediates the expression of Skp2 protein. | Cell Death Discovery

Fig. 4: USP14 mediates the expression of Skp2 protein.

From: USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein

Fig. 4: USP14 mediates the expression of Skp2 protein.The alternative text for this image may have been generated using AI.

A VSMCs were exposed to IU1 and/or PDGF-BB, followed by Western blot assay for Skp2 expression. B A7r5 cells were transfected with USP14 siRNA and/or PDGF-BB. Western blot assay was conducted to detect Skp2 expression. C, D HASMCs were treated with CHX and IU1/USP14 siRNA+CHX for 2, 4, and 6 h, respectively. Western blot assay was performed to assess Skp2 protein. E HEK293T cells were transfected with MYC-Skp2 or MYC-Skp2+FLAG-USP14 for 48 h and treated with CHX, followed by western blot assay. F The intensity of Skp2 band was quantified by Image J. G, H HASMCs were treated with IU1/USP14 siRNA and/or PDGF-BB. Immunofluorescence staining with USP14 was assessed by confocal microscopy.

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