Fig. 5: Interaction between Skp2 and USP14. | Cell Death Discovery

Fig. 5: Interaction between Skp2 and USP14.

From: USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein

Fig. 5: Interaction between Skp2 and USP14.The alternative text for this image may have been generated using AI.

A Protein lysate was obtained from HASMCs. IP with anti-Skp2 antibody or anti-USP14 antibody, immunoblot with USP14 and Skp2. B HEK293T cells were transfected with His-tagged USP14 and MYC-tagged Skp2. IP with MYC, IB with His and MYC. C HASMCs transfected with MYC-tagged Skp2/MYC-USP14 were stained with anti-MYC and anti-USP14/Skp2 antibodies, followed by confocal microscopy. D HASMCs were exposed to USP14 siRNA for 48 h, followed by IP with Skp2 and IB for Ub and K48 proteins. E HASMCs were treated with IU1. IP with Skp2 and IB with K48. F HEK293T cells were transfected with HA-Ub, His-USP14 (WT), His-USP14 (C114A), and Myc-Skp2. IP with MYC and IB with HA.

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