Fig. 3: N-terminal region of LIMA1 is required for its association with RNF40.
From: LIMA1 links the E3 ubiquitin ligase RNF40 to lipid metabolism
A Flag-LIMA1 or L25I and RNF40-Myc were co-expressed in HEK293T cells followed by co-immunoprecipitation with Anti-Flag antibody and immunoblotting analysis. B Nuclear and cytoplasmic protein extraction was performed and the separated proteins were subjected for immunoblotting analysis. C Co-IP was performed for RNF40 in Hep3B cells and LIMA1 protein was detected by immunoblotting. D Schematic diagram of full-length LIMA1 and their various deletion mutants. E HEK293T cells were co-transfected with RNF40-Myc and different LIMA1 mutants, followed by immunoprecipitation with anti-Myc antibody and immunoblotting analysis.
