Fig. 5: Combination of D-2HG and TMZ triggers synergistic anti-glioma activity.

A IC50 curves of TMZ in U251 cells at 24 h, 48 h, and 72 h, respectively. B, C Isobologram analysis shows the synergistic cytotoxic effect of D-2HG plus TMZ. CI < 1 indicates synergy. D Cell viability and E Cell toxicity of U251 treated with four combinations of D-2HG and TMZ.