Fig. 2: ATP6V0A2 overexpression alleviates EIC in vitro. | Cell Death Discovery

Fig. 2: ATP6V0A2 overexpression alleviates EIC in vitro.

From: Epirubicin induces cardiotoxicity through disrupting ATP6V0A2-dependent lysosomal acidification and triggering ferroptosis in cardiomyocytes

Fig. 2

HL-1 cardiomyocytes were transfected with plasmids of scramble or ATP6V0A2 for 48 h, and then treated with DMSO or EPI (2 μM) for 12 h. A–C Immunoblotting images of ATP6V0A2 and ANP proteins in HL-1 cardiomyocytes in the indicated groups. D Cell viability was detected in HL-1 cardiomyocytes treated as indicated. E LDH release in the medium of HL-1 cardiomyocytes in the indicated groups was detected. Two-way ANOVA and subsequent Tukey tests were performed for statistical analysis. ***p < 0.001, **p < 0.01, *p < 0.05.

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