Fig. 4: Ferroptosis is triggered in epirubicin induced cardiotoxicity.
HL-1 cardiomyocytes were treated with DMSO, erastin(10 μM), EPI(2 μM) or Fer-1 (50 μM) for 12 h. Fer-1 was pretreated for 2 h before the establishment of EIC. A Cell viability was measured by CCK-8 of HL-1 cardiomyocytes treated as indicated. B LDH release in the medium of HL-1 cardiomyocytes was detected in the indicated groups. The mRNA levels of PTGS2 (C) and NOX1 (D) in HL-1 cardiomyocytes in the indicated groups. E–G Immunoblotting images of PTGS2 and NOX1 proteins of HL-1 cardiomyocytes in the indicated groups. H, I DCFH-DA probe was used to detect the relative ROS level of HL-1 cardiomyocytes in the indicated groups flow cytometry and the quantitative analysis of DCF (FL1) fluorescence are shown. J, K BODIPY581/591- C11 probe was used to detected lipid peroxidation level in HL-1 cardiomyocytes treated with indicated chemicals by flow cytometry and the quantitative analysis of BODIPY581/591-C11 (FL1) fluorescence are shown. L The relative GSH level of HL-1cardiomyocytes was detected in the indicated groups. One-way ANOVA followed by the Tukey post hoc test was used to compare indicated mice with saline-treated mice. ***p < 0.001, *p < 0.05.
