Fig. 4: Th1 differentiation after p38 MAPK inhibition. | Cell Death Discovery

Fig. 4: Th1 differentiation after p38 MAPK inhibition.

From: Ca2+ signals are essential for T-cell proliferation, while Zn2+ signals are necessary for T helper cell 1 differentiation

Fig. 4

PBMC were preincubated for 30 min with the p38 MAPK inhibitor SB202190 (10 µM) and then stimulated with pyrithione and thapsigargin at the indicated concentrations. A Protein expression of the transcription factor T-bet was examined by western blot 48 h after stimulation and normalized to β-actin. The control data shown in (A) are also included in the data shown in Fig. 3E. B IRF-1 expression was also analyzed by qPCR after 3 h and relative mRNA expression was determined by 2−ΔΔCt method. The control data shown in (B) are also included in the data shown in Fig. 3F. C After 48 h, IFN-γ was measured by ELISA. The control data shown in (C) are partially included in the data shown in Fig. 3D. Data are presented as mean + SEM with n = 6 (A), n = 7 (B) and n = 7 (C) experiments. Statistical significance was determined by Friedman test with Dunn’s multiple comparisons test (A, B) or by one-way ANOVA with Sidak’s multiple comparisons test (C) (*p < 0.05; **p < 0.01; ***p < 0.001).

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