Fig. 6: Zinc and calcium signals do not induce Th2 and Th17 differentiation. | Cell Death Discovery

Fig. 6: Zinc and calcium signals do not induce Th2 and Th17 differentiation.

From: Ca2+ signals are essential for T-cell proliferation, while Zn2+ signals are necessary for T helper cell 1 differentiation

Fig. 6

PBMC were stimulated at the same time with pyrithione and thapsigargin at the indicated concentrations and differentiation into Th2 and Th17 was determined. A 48 h after stimulation, IL-10 (Th2) and B IL-17 (Th17) were measured by ELISA. C Protein expression of the transcription factor GATA-3 (Th2) and D RORC2 (Th17) were examined by western blot 48 h after stimulation and normalized to β-actin. For each a representative western blot is shown. Data are presented as mean + SEM with n = 3 (A, B), n = 5 (C, D) experiments. Statistical significance was tested by one-way ANOVA with Tukey’s multiple comparisons test (A, B) or Friedman test with Dunn’s multiple comparisons test (D), but no significances were found.

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