Fig. 3: HDAC3 depletion in combination with IR reduces stemness and tumorigenicity in vitro and suppresses growth in vivo. | Cell Death Discovery

Fig. 3: HDAC3 depletion in combination with IR reduces stemness and tumorigenicity in vitro and suppresses growth in vivo.

From: HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks

Fig. 3

A Representative light microscopy pictures of cancer stem cell formation assay on RH30 and RH4 cells infected with either sgControl (sgCTR) or sgRNA against HDAC3 (sgHDAC3) at 72 h post-infection and then treated or not with 6 Gy of IR. Scale bar = 50 μm. B Histogram depicts the quantification of rhabdospheres number per field. n = 3 independent experiments, data presented as mean values ± SD, one-way ANOVA. C Representative western blot (n = 3) of RH30 and RH4 cells treated as in (A) reporting MYCN, pERK and ERK protein levels. Vinculin was used as loading control. D Tumor volume of sgCTR (n = 9), sgHDAC3 (n = 6) of RH30 cells xenografts assessed by caliper measurement represented in mm3 followed for 48 days post-inoculation. 13 days after inoculation mice were irradiated each 3 days for 5 times with a single dose of 2 Gy of IR. Data presented as mean values ± SD, two-way ANOVA. E Tumor weight of transplanted RH30 cell xenografts treated as in (D). Data presented as mean values ± SD, two-way ANOVA.

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