Fig. 5: MC4448 is a new potent and highly selective HDAC3 inhibitor. | Cell Death Discovery

Fig. 5: MC4448 is a new potent and highly selective HDAC3 inhibitor.

From: HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks

Fig. 5

A Biochemical data of MC4448 against HDAC1, −3, −4, −6 and −8 at three fixed doses (0.01, 0.3, and 7 µM). B Dose-response curves of RH30 and RH4 cells treated with increasing concentration of MC4448. Graph represents the mean of three independent experiment ±SEM. C Representative western blot (n = 3) of RH30 and RH4 treated with MC4448 IC50 for 24 h showing levels of acetylated H3. Vinculin was used as loading control. D Growth curve analysis of RH30 and RH4 cells treated as in (C). n = 3 independent experiments, data presented as mean values ± SD, Student’s two-tailed t test. E Heatmap depicting dose response effect of MC4448 treatment for 72 h on FP-RMS cells (RH30 and RH4), and Normal Human Skeletal Muscle Myoblast (HSMM) and Normal Fetal Human Lung Fibroblast (MRC5).

Back to article page