Fig. 7: MC4448 treatment in combination with IR reduces stemness and counteracts IR-dependent induction of MYCN and p-ERK. | Cell Death Discovery

Fig. 7: MC4448 treatment in combination with IR reduces stemness and counteracts IR-dependent induction of MYCN and p-ERK.

From: HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaks

Fig. 7

A Representative light microscopy pictures of cancer stem cell formation assay on RH30 and RH4 cells treated with either DMSO or MC4448 IC50 for 24 h and then treated or not with 6 Gy of IR and grown for 2 weeks. Scale bar = 50 μm. B Histogram depicts the quantification of rhabdosphere numbers per field. n = 3 independent experiments, data presented as mean values ± SD, one-way ANOVA. C Representative western blot (n = 3) of RH30 and RH4 cells treated as in A reporting MYCN, pERK and ERK protein levels. Vinculin was used as loading control.

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