Fig. 2: HDAC1 and SIRT1 are overexpressed in TNBC and inhibit miRNAs-449 expression by histone deacetylation. | Cell Death Discovery

Fig. 2: HDAC1 and SIRT1 are overexpressed in TNBC and inhibit miRNAs-449 expression by histone deacetylation.

From: MiRNA-449 family is epigenetically repressed and sensitizes to doxorubicin through ACSL4 downregulation in triple-negative breast cancer

Fig. 2

HDAC1 (A) and SIRT1 (B) expression were analyzed by RT-qPCR (mean ± SD) (n = 3)and Western blot (C) in TNBC cell lines (MDA-MB-231 and MDA-MB-436) and the non-tumor immortalized line MCF10A. MiRNA-449 family (miRNA-449a, miRNA-449b-5p, and miRNA-449c-5p) expression was analyzed by RT-qPCR in TNBC cell lines (MDA-MB- 231 and MDA-MB-436) after siHDAC1 (D) or siSIRT1 (E) transfection (mean ± SD) (n = 3). Acetyl-H3 (Ac-H3) and H3 expression was analyzed by Western blot in TNBC cell lines after TSA (F) (10 nM, 24 h) or NAM (G) (300 µM, 24 h) treatment. MiRNA-449 family expression was analyzed by RT-qPCR in TNBC cell lines after TSA (H) (10 nM, 24 h) or NAM (I) (300 µM, 24 h) treatment (mean ± SD) (n = 3). *p < 0.0500, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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