Fig. 6: MiRNAs-449 overexpression sensitizes to doxorubicin through ACSL4 downregulation. | Cell Death Discovery

Fig. 6: MiRNAs-449 overexpression sensitizes to doxorubicin through ACSL4 downregulation.

From: MiRNA-449 family is epigenetically repressed and sensitizes to doxorubicin through ACSL4 downregulation in triple-negative breast cancer

Fig. 6

ACSL4 expression was analyzed by RT-qPCR (mean ± SD) (n = 3) (A) and Western blot (B) in MDA-MB-231, MDA-MB-436, and MDA-MB-231R cell lines after doxorubicin treatment (1 µM, 48 h). Cell cytotoxicity was analyzed by WST-1 to determine the IC50 value (mean ± SD) (n = 3). MDA-MB-231, MDA-MB-436, and MDA-MB-231R cell lines were transfected with miRNAs-449 mimics (C), siACSL4#1 (D), or siACSL4#2 (E), and different doses of doxorubicin. *p < 0.0500, **p < 0.01, ***p < 0.001, ****p < 0.0001. DOX; doxorubicin.

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