Fig. 2: Impact of IGFBP-2 on Proliferation and Migration of GBM Cells.
From: SVIP reduces IGFBP-2 expression and inhibits glioblastoma progression via stabilizing PTEN
A–D The efficiency of IGFBP-2 overexpression and knockdown was evaluated using Western blot and qRT-PCR techniques. E, F In U87-MG cells, IGFBP-2 was respectively overexpressed or knocked down, followed by cell viability assessment using the CCK-8 assay. G, H In U87-MG cells, IGFBP-2 was respectively overexpressed and knocked down, followed by the assessment of cell migration ability using the Transwell assay. I, J In U87-MG cells, IGFBP-2 was respectively overexpressed and knocked down, followed by the assessment of cell proliferation using EdU labeling. Statistical analysis: data were quantified as mean ± SD, n ≥ 3, two tailed student’s t test, P < 0.01, **; P < 0.001, ***; P < 0.0001, ****.
