Fig. 3: STUB1 and SVIP Regulate the Expression of PTEN and IGFBP-2 and the Biological Functions of GBM Cells.

A The CGGA database was utilized to analyze the correlation between the expression levels of STUB1 and IGFBP-2 across various grades of gliomas. B The CGGA database was utilized to analyze the correlation between the expression levels of STUB1 and SVIP across various grades of gliomas. C, D In LN229 cells, transfection was performed with STUB1 or SVIP overexpression plasmids separately. Subsequently, Western blot analysis was conducted to assess the expression levels of STUB1, SVIP, PTEN, and IGFBP-2. E U87-MG cells were co-transfected with PTEN-GFP overexpression plasmid and empty vector, PTEN-GFP and STUB1 overexpression plasmids, respectively. Western blot analysis was conducted to evaluate the protein expression levels of STUB1, PTEN, and IGFBP-2 in the U87-MG cells. F U87-MG cells were co-transfected with PTEN-GFP overexpression plasmid and empty vector, PTEN-GFP and SVIP overexpression plasmids, respectively. Western blot analysis was conducted to evaluate the protein expression levels of SVIP, PTEN, and IGFBP-2 in the U87-MG cells. G, I In LN229 cells, plasmid transfection was conducted as described above. The migration ability of the cells was evaluated using the Transwell assay, while the proliferation capacity was assessed through EdU staining. H, J In U87-MG cells, plasmid transfection was conducted as described above. The migration ability of the cells was evaluated using the Transwell assay, while the proliferation capacity was assessed through EdU staining. Statistical analysis: data were quantified as mean ± SD, n ≥ 3, two tailed student’s t test, P＜0.05, *; P＜0.01,**; P＜0.001,***.