Fig. 6: G6PD is targeted by METTL14-mediated m6A modification.
From: METTL14-mediated m6A mRNA modification of G6PD promotes lung adenocarcinoma
A Consensus motif identified in LUAD cells by MeRIP-seq. B Volcano plot showing DEGs identified by RNA-seq in LUAD cells with or without METTL14 knockdown. C–E Relative abundance of m6A peaks in G6PD mRNA analyzed by MeRIP-seq in LUAD cells. F Enrichment binding sites of m6A signals in G6PD mRNA. G Western blot and analysis of G6PD protein levels in LUAD cells (n = 3). H RT-qPCR analysis of G6PD expression in LUAD cells (n = 3). I Sequence motifs in m6A-modified sites of G6PD mutants. J, K Sequence motifs and cellular luciferase assay in G6PD cells or 3’-UTR mutant LUAD cells with or without METTL14 knockdown (n = 3). ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.
