Fig. 5: HnRNPA2B1 regulates Tert and promotes CM proliferation. | Cell Death Discovery

Fig. 5: HnRNPA2B1 regulates Tert and promotes CM proliferation.

From: Tert promotes cardiac regenerative repair after MI through alleviating ROS-induced DNA damage response in cardiomyocyte

Fig. 5

A SDS-PAGE images of proteins captured by biotin-labeled Tert probes. There were significant variations in bands between the Target and IgG groups. B The basal expression of hnRNPA2B1 in P1 CMs and P7 CMs (*P < 0.05 vs. P1; n = 3 cell samples). C, D Western blotting assay and quantitative analysis of hnRNPA2B1 and Tert in P7 CMs with hnRNPA2B1 overexpression and P1 CMs with hnRNPA2B1 knockdown (*P < 0.05 vs. ADV-NC or ADV-sh-NC; n = 4 cell samples, the experiment was replicated in the laboratory a total of three times). E, F Western blotting assay and quantitative analysis of hnRNPA2B1 and Tert in hearts from P7 mice with hnRNPA2B1 overexpression and P1 mice with hnRNPA2B1 knockdown (*P < 0.05 vs. ADV-NC or ADV-sh-NC; n = 4 cell samples, the experiment was replicated in the laboratory a total of three times). G, H Immunohistochemical assay and quantitative analysis of Tert in cardiac tissues from P7 mice with hnRNPA2B1 overexpression and P1 mice with hnRNPA2B1 knockdown (*P < 0.05 vs. ADV-NC or ADV-sh-NC; n = 6 slices from 6 mice per group). I, J Immunofluorescence images and quantitative analysis of telomere length in P7 CMs with hnRNPA2B1 overexpression and P1 CMs with hnRNPA2B1 knockdown (*P < 0.05 vs. ADV-NC or ADV-sh-NC; 544 CMs from 18 images of 6 mice in ADV-hnRNPA2B1 group; 579 CMs from 18 images of 6 mice in ADV-NC group; 532 CMs from 18 images of 6 mice in ADV-sh-hnRNPA2B1 group; 497 CMs from 18 images of 6 mice in ADV-sh-NC group). K, L Immunofluorescence images and quantitative analysis of Edu in P7 CMs with hnRNPA2B1 overexpression and P1 CMs with hnRNPA2B1 knockdown (Edu+ CMs are indicated by arrows, *P < 0.05 vs. ADV-NC or ADV-sh-NC; 498 CMs from 18 images of 6 mice in ADV-hnRNPA2B1 group; 491 CMs from 18 images of 6 mice in ADV-NC group; 501 CMs from 18 images of 6 mice in ADV-sh-hnRNPA2B1 group; 439 CMs from 18 images of 6 mice in ADV-sh-NC group). Error bars indicate SD of three biological repeats in B, that of four biological repeats in C–F and that of six biological repeats in G–L. An unpaired t test in B/I–L, two-way ANOVA in C–F were utilized to determine the statistical significance.

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