Fig. 5: MDH2 interacts with FSP1 and promotes its ubiquitination. | Cell Death Discovery

Fig. 5: MDH2 interacts with FSP1 and promotes its ubiquitination.

From: MDH2 regulates the sensitivity of clear cell renal cell carcinoma to ferroptosis through its interaction with FSP1

Fig. 5

A mRNA expression levels of ferroptosis key gene in MDH2 overexpressing and knockout ccRCC cells. The expression level of β-Actin was used as normalized control. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. B Western blot was performed to detect the stability of FSP1 protein in MDH2 knockout and negative control cells. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. C Co-IP detecting the interaction between MDH2 and FSP1 in 786-O cells. D WB detection of FSP1 ubiquitination in 786-O cells. The statistical analysis comes from biological replicates and the experiment was repeated three times. Data was presented as mean ± SD. E Protein expression of FSP1 from the CPTAC ccRCC dataset. F Investigate the correlation between MDH2 and FSP1 in tissue samples from ccRCC patients. (*P < 0.05, **P < 0.01, ***P < 0.001, and ns not significant.).

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