Fig. 1: The correlative changes between NSF activity and expressions of STX17 and VAMP8 were revealed after ischemia.

Western blot (A, B) showed that NSF activity was drastically inhibited, while the expressions of STX17 and VAMP8 was reduced in penumbral areas 48 h after MCAO. Immunofluorescence (C) showed that the inactivated NSF (D) and decreased expressions of STX17 (F) were predominantly occurred in neurons at the penumbra (E, G). The expression of VAMP8 (H, I) was also significantly reduced. n = 6. *p < 0.05, **P < 0.01, ***p < 0.001; ns indicates that the data are not statistically different.