Fig. 2: IL-1β, pyroptosis-released inflammatory factor, enhances T-cell proliferation and activation. | Cell Death Discovery

Fig. 2: IL-1β, pyroptosis-released inflammatory factor, enhances T-cell proliferation and activation.

From: Pyroptosis of oral keratinocyte contributes to energy metabolic reprogramming of T cells in oral lichen planus via OPA1-mediated mitochondrial fusion

Fig. 2

A CellTrace™ CFSE-labeled OLP-T cells conditioned co-cultured with culture supernatant from indicated primary epithelial cells, analyzed using flow cytometry, proliferation rates of OLP-T cells in the indicated groups were quantitatively analyzed. The blue represents the primary peak from co-culturing with fresh epithelial cell culture medium, without CD3/CD28 stimulation, and the brown represents the proliferation peak under the indicated conditions. B The mRNA levels of inflammatory factors released after T-cell activation, detected after conditioned co-culturing with culture supernatant from indicated primary epithelial cells. C CellTrace™ CFSE-labeled OLP-T cells analyzed using flow cytometry, under treatment with exogenous IL-1β, with or without IL-1Ra, proliferation rates were quantitatively analyzed. The blue represents the primary peak without CD3/28 stimulation, the brown represents the proliferation peak under the indicated conditions. D The mRNA levels of inflammatory factors released after T-cell activation, under treatment with exogenous IL-1β, with or without IL-1Ra. *P < 0.05. **P < 0.01. ***P < 0.001. OLP-T cells T cells from peripheral blood of patients with OLP, αIL-1β IL-1β neutralizing antibody; IL-1Ra inhibitor of IL-1β receptor 1.

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