Fig. 1: 4-AP expedited burn injury closure and enhanced skin regeneration.

A Schematic illustration of experimental design to test the beneficial therapeutic effect of 4-AP in the C57BL/6 mouse burned model. B Representative digital images of burn healing in saline and 4-AP (2 mg/kg/IP, single dose immediately after surgery and until day 21) treated mice at 0, 3, 5, 7, 10, 14, 18, and 21 days post-burning. Scale bar = 5 mm. C Percent burn wound closure at each time point relative to the initial burn wound area in control and 4-AP-treated mice. n = 6 animals per group, with one burn wound per animal. D Representative images of H&E-stained sections of full-thickness burn of saline and 4-AP treated skin tissue on days 3, 7, and 21. Scale bar = 100 µm, n = 4 skin tissues per group. E, F Quantification of burn wound length and area in H&E-stained skin burn sections using ImageJ software on days 3, 7, and 21. n = 4 skin tissues per group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (*P < 0.05, and **P < 0.0021 vs. saline group) and compared using two-tailed, unpaired t-tests.