Fig. 5: 4-AP attenuated pro-apoptosis and accelerated anti-apoptosis effects following skin burn. | Cell Death Discovery

Fig. 5: 4-AP attenuated pro-apoptosis and accelerated anti-apoptosis effects following skin burn.

From: 4-aminopyridine attenuates inflammation and apoptosis and increases angiogenesis to promote skin regeneration following a burn injury in mice

Fig. 5

A, B Representative images of DAB-TUNEL staining of apoptosis and quantitative results of TUNEL positive cells in saline and 4-AP treated skin burn tissues on days 3 and 7. n = 4 skin tissues per group. C qRT-PCR data shows that 4-AP treatment significantly attenuated pro-apoptosis genes (BAX, caspase-9, and caspase-3) and upregulated anti-apoptosis genes (BCL2) compared to the saline group on days 3 and 7. n = 3 skin tissues per group. D, E Western blotting images and quantitative results showed that 4-AP treatment significantly attenuated expressions of pro-apoptosis protein (BAX) and upregulated anti-apoptosis proteins (BCL2 and BCL-XL) as compared to the saline group on days 3 and 7. n = 3 skin tissues per group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (*P < 0.05, **P < 0.0021, and ***P < 0.0002 vs. saline group) and compared using two-tailed, unpaired t-tests.

Back to article page