Fig. 6: 4-AP accelerated epithelization and keratinocytes terminal differentiation following skin burn. | Cell Death Discovery

Fig. 6: 4-AP accelerated epithelization and keratinocytes terminal differentiation following skin burn.

From: 4-aminopyridine attenuates inflammation and apoptosis and increases angiogenesis to promote skin regeneration following a burn injury in mice

Fig. 6

A, B Representative images and quantitative results of IF staining of keratinocyte markers (K10; terminal differentiation and K14; proliferative) in saline and 4-AP treated skin burn tissues on days 3 and 7. Scale bar = 100 µm. n = 4 skin tissues per group. C, D Western blotting and (E) qRT-PCR quantitative results show that 4-AP treatment significantly increased keratin markers protein and gene (K10 and K14) expressions compared to the saline group on days 3 and 7. n = 3 skin tissues per group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (*P < 0.05, **P < 0.0021, and ***P < 0.0002 vs. saline group) and compared using two-tailed, unpaired t-tests.

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