Fig. 7: 4-AP increased fibroblast to myofibroblast transformation following skin burn. | Cell Death Discovery

Fig. 7: 4-AP increased fibroblast to myofibroblast transformation following skin burn.

From: 4-aminopyridine attenuates inflammation and apoptosis and increases angiogenesis to promote skin regeneration following a burn injury in mice

Fig. 7: 4-AP increased fibroblast to myofibroblast transformation following skin burn.

A, B Representative images and quantitative results of IF staining of vimentin (fibroblasts) and α-SMA (myofibroblasts) in saline and 4-AP treated skin burn tissues on days 3 and 7. Scale bar = 100 µm. n = 4 skin tissues per group. C qRT-PCR data shows that 4-AP treatment significantly increased the expression of TGFβ, vimentin, α-SMA, and FGF genes compared to the saline group on days 7 and 21. n = 3 skin tissues per group. D, E Western blotting images and quantitative results showed that 4-AP treatment significantly increased the expression of TGFβ, vimentin, and α-SMA proteins compared to the saline group on days 7 and 21. n = 3 skin tissues per group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (*P < 0.05, **P < 0.0021, and ***P < 0.0002 vs. saline group) and compared using two-tailed, unpaired t-tests.

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