Fig. 8: 4-AP advanced skin burns wound repair by increasing collagen and MMP expressions. | Cell Death Discovery

Fig. 8: 4-AP advanced skin burns wound repair by increasing collagen and MMP expressions.

From: 4-aminopyridine attenuates inflammation and apoptosis and increases angiogenesis to promote skin regeneration following a burn injury in mice

Fig. 8

A, B Representative images and quantitative results of Herovici’s staining of collagen III/I in saline and 4-AP treated skin burn tissues on days 7 and 21. n = 4 skin tissues per group. C qRT-PCR data shows that 4-AP treatment significantly increased the expression of collagen-III/I and MM9/3 genes compared to the saline group on days 7 and 21. n = 3 skin tissues per group. D, E Western blotting images and quantitative results showed that 4-AP treatment significantly increased collagen-III/I protein expressions compared to the saline group on day 21. n = 3 skin tissues per group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (*P < 0.05, **P < 0.0021, and P*** < 0.0002 vs. saline group) and compared using two-tailed, unpaired t-tests. Schematic illustration of early (F) and late (G) phases of skin burn healing. 4-AP expedites burn wound healing via controlling multi-cellular events of (i) macrophage, (ii) apoptosis, (iii) angiogenesis, (iv) keratinocyte, and (v) fibroblast. During the early phase (days 3 and 7) of burn wound healing, 4-AP significantly mitigates inflammation via Orai1-pSTA6 signaling and apoptosis and enhances angiogenesis, keratin, and fibroblast differentiation. In the later phase (day 21), 4-AP promotes wound closure by accelerating keratinocyte differentiation and accelerating the remodeling of the ECM via stimulating fibroblast and collagen differentiation.

Back to article page