Fig. 3: ASO-circMETTL3 treatment ameliorated hypercytokinemia and blood metrics associated with sHLH. | Cell Death Discovery

Fig. 3: ASO-circMETTL3 treatment ameliorated hypercytokinemia and blood metrics associated with sHLH.

From: CircMETTL3-156aa reshapes the glycolytic metabolism of macrophages to promote M1 polarization and induce cytokine storms in sHLH

Fig. 3

A Treatment schedules of different mouse groups including saline, ASO-NC and ASO-CircMETTL3 for IL-10R antibody and CPG-induced sHLH mouse models. B The change of body weight from all groups for IL-10R antibody and CPG-induced sHLH mouse models. C, D Image of spleen, spleen% of body weight and Liver% of body weight from all the groups. E–G The effect of ASO-circMETTL3 on sHLH were investigated by blood routine test indicated by RBC (E), WBC (F), Hb (G) (n = 5). Representative data from one experiment out of three independent experiments. n = 5, each symbol represents one biological replicates. Data have been represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001 (B–G). H. FACS analysis of the percentage CD45 + /CD3,CD19-/CD11b + /CD86+ macrophages in blood from different treatment groups. I. Analysis of the average fluorescence intensity of CD86. Error bars are mean ± SD for n = 3 biological replicates.***p < 0.001,****p < 0.0001. J. The effect of ASO-CircMETTL3 on cytokine storm were assessed by quantification of serum levels of multiple cytokines including IFN-γ, TNF-α, IL-12p70, IL-1β, CXCL10, IL-10, IL-6 and GMCSF (n = 5). Representative data from one experiment out of two independent experiments. n = 5, each symbol represents one biological replicates. Data have been represented as mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001. K Treatment schedules of different mouse groups including saline, ASO-NC and ASO-CircMETTL3 for poly (I:C) and LPS-induced lethal sHLH model. L–N The effect of ASO-circMETTL3 on sHLH were investigated by blood routine test indicated by WBC (L), PLT (M) and lactate dehydrogenase (LDH) (N) (n = 5). O. Flow cytometry was used to semi-quantitatively evaluate the levels of cytokines including IFN-γ, TNF-α, IL-12p70, IL-1β, CXCL10, IL-10, IL-6, and GM-CSF in all the groups serum (n = 5). Representative data from one experiment out of two independent experiments. n = 5, each symbol represents one individual mouse. Data have been represented as mean ± SD, *p < 0.05, **p < 0.01 and ****p < 0.0001 (L–O). P Survival curves of poly (I:C) and LPS-induced sHLH mouse model treated with saline (Naive group) or ASO-NC (HLH-ASO-NC group) or ASO-CircMETTL3 (HLH-ASO-Circ group), (n = 5, each symbol represents one individual mouse from one independent experiment. HLH-ASO-NC group was significantly different compared to HLH-ASO-Circ group, p = 0.0197).

Back to article page