Fig. 1: Aurora-B is SUMOylated by SUMO-2/3 in mouse oocytes. | Cell Death Discovery

Fig. 1: Aurora-B is SUMOylated by SUMO-2/3 in mouse oocytes.

From: Mutation of the SUMOylation site of Aurora-B disrupts spindle formation and chromosome alignment in oocytes

Fig. 1: Aurora-B is SUMOylated by SUMO-2/3 in mouse oocytes.

A Subcellular localization of Aurora-B and SUMO-2/3 in mouse oocytes at different phases. Mouse oocytes were matured and harvested in vitro at the following stages: GV (0 h), GVBD (4 h), pre-MI (6 h), MI (8 h), AI (10 h), and MII (16 h). Rabbit anti-p-Aurora-B (green), rat anti-SUMO-2/3 (red), and DAPI-labeled DNA (blue) were used. Scale bar: 5 μm. B Co-immunoprecipitation was performed with anti-Aurora-B and detected by WB using an anti-SUMO-2/3 antibody. C Co-immunoprecipitation was performed with anti-SUMO-2/3 and detected by WB using anti-Aurora-B.

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