Fig. 2: NS is upregulated following CoCl2-induced hypoxia and reoxygenation in H9C2 cells. | Cell Death Discovery

Fig. 2: NS is upregulated following CoCl2-induced hypoxia and reoxygenation in H9C2 cells.

From: Myocardial ischemia-reperfusion injury upregulates nucleostemin expression via HIF-1α and c-Jun pathways and alleviates apoptosis by promoting autophagy

Fig. 2: NS is upregulated following CoCl2-induced hypoxia and reoxygenation in H9C2 cells.The alternative text for this image may have been generated using AI.

A Assessment of CoCl2-induced H9C2 cell death at various concentrations using CCK8. B Volcano plot analysis of RNA-seq data in H9C2 cells between hypoxia 12 h/reperfusion 6 h and sham samples (n = 3, FDR < 0.05 and |log2FC| > 0.58). C, D Western blot assays depicting the expression of NS after 12 h of hypoxia induced by 500 μM CoCl2 and reperfusion for 0, 2, 4, 6, 12, and 24 h (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, vs. NC group.

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