Fig. 3: Effects of exendin-4 on proliferation of mouse reformed islets. | Cell Death Discovery

Fig. 3: Effects of exendin-4 on proliferation of mouse reformed islets.

From: Reformed islets: a long-term primary cell platform for exploring mouse and human islet biology

Fig. 3

The reformed (A) and native islets (C) were exposed to exendin-4 for 30 h and immunoprobed with antibodies against insulin (INS; green) and Ki67 (red). DAPI is shown in blue in merged images. Scale bar is 20 µm. Percentage calculation of Ki67+DAPI+ cells within the reformed islets and native islets were performed using the cell counter plugin in ImageJ software (B, D, E). Data are expressed as mean ± SEM, n = 5 observations (10–15 of islets per experiment) from two separate experiments. *p < 0.05 relative to the control samples, One-way ANOVA, Dunnett’s multiple comparisons test.

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