Fig. 1: Both thiomuscimol and muscimol inhibit pyroptotic lysis, but thiomuscimol alone rescues cell viability. | Cell Death Discovery

Fig. 1: Both thiomuscimol and muscimol inhibit pyroptotic lysis, but thiomuscimol alone rescues cell viability.

From: Broad-spectrum inflammasome inhibition by thiomuscimol

Fig. 1

Structures of muscimol and thiomuscimol, with the single oxygen to sulfur replacement highlighted (A). Bone marrow-derived macrophages (BMDM) were treated with Salmonella, lethal toxin or lysis buffer in the presence of the indicated concentrations of thiomuscimol or muscimol (B–D). LDH released during pyroptotic lysis was measured (B, C). Viability was assessed by measuring cellular ATP content (D). Data are means±SD, n = 3 replicates, representative of two independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001 (Ordinary one-way ANOVA with Dunnett’s multiple comparison test) indicates significance compared to vehicle control. Curly brackets indicate shared significance value.

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