Fig. 3: Thiomuscimol inhibits ASC speck formation and To-PRO-3 uptake in a dose-dependent manner.
From: Broad-spectrum inflammasome inhibition by thiomuscimol
ASC-citrine expressing BMDM were infected with Salmonella for 90 min in the presence of muscimol or thiomuscimol as indicated. Localization of the inflammasome adapter ASC (yellow) and uptake of the small membrane-impermeant nuclear dye To-PRO-3 (red) were assessed (A–C). Data are means ± SD, n = 3 replicates, representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Ordinary one-way ANOVA with Dunnett’s multiple comparison test) indicates significance compared to infection in the absence of thiomuscimol. Curly brackets indicate shared significance value.
