Fig. 5: Thiomuscimol inhibits Pyrin and AIM2 induced ASC speck formation.
From: Broad-spectrum inflammasome inhibition by thiomuscimol
ASC-citrine expressing BMDM were primed with LPS (500 ng/mL, 3 h) and treated with TcdA or poly(dA:dT) for 3 h to activate pyrin or AIM2 respectively in the presence of thiomuscimol as indicated. ASC speck formation was kinetically measured over time to monitor inflammasome formation (A–C). Data are means ± SD, n = 3 replicates, representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (Unpaired T-test) indicates significance compared to pyrin activation in the absence of thiomuscimol.
