Fig. 1: TRIM47 promotes HCC cells migration and invasion.
A Analysis of TRIM47 mRNA expression in HCC primary tumor tissues and normal liver tissues from TCGA database. *P < 0.05. B Analysis of TRIM47 mRNA expression in different tumor stages of HCC patients from TCGA database. *P < 0.05. C Kaplan–Meier survival analysis of overall survival (OS) stratified by TRIM47 expression in HCC tumor tissues from TCGA database. P = 0.0375. D Western blot analysis of TRIM47 protein levels in TRIM47 stably knockdown (shTRIM47#1 and shTRIM47#2) or control (shNC) SMMC7721 cells. E, F The migration assay (upper panel) and invasion assay (bottom panel) of SMMC7721 cells with TRIM47 stably knockdown (E). The average number of cells per field were calculated (F). Scale bars, 50 μm. Data are shown as mean ± SD. n = 3 samples per group, four fields per sample. **P < 0.01, ***P < 0.001. G Western blot analysis of TRIM47 protein levels in SMMC7721 cells transiently transfected with Vector or FLAG-TRIM47. H, I The migration assay of SMMC7721 cells transiently transfected with Vector or FLAG-TRIM47 (H). The average number of cells per field were calculated (I). Scale bars, 50 μm. Data are shown as mean ± SD. n = 3 samples per group, four fields per sample. **, P < 0.01. J, K 2 × 106 TRIM47 stably knockdown (shTRIM47#1 and shTRIM47#2) or control (shNC) SMMC7721 cells were injected into nude mice (n = 5 per group) via tail vain. The representative haematoxylin and eosin (H&E) images (J) and quantification of metastatic foci in lungs (K) were shown. Black arrows indicated the metastatic foci. Scale bars, 100 μm. Data are shown as mean ± SD. ***P < 0.001.
