Fig. 2: BAP1 and LDHA have protein-to-protein interactions. | Cell Death Discovery

Fig. 2: BAP1 and LDHA have protein-to-protein interactions.

From: BAP1 inactivation promotes lactate production by leveraging the subcellular localization of LDHA in melanoma

Fig. 2

A Heat map showing the mean expression values of genes related to glucose metabolism, data from RNA-seq data of BAP1 knockdown group and control group in MEL290, MUM2B cell lines. B Fluorescence quantitative PCR was performed on differentially expressed genes, and the samples were MEL290 cell lines. C Western blot of differentially expressed genes to detect protein expression levels in the BAP1 knockdown and control groups of MEL290 cell lines. D Immunoprecipitation of BAP1 was performed on the MEL290 cell line, and protein characterization was performed using triple quadrupole tandem mass spectrometry and compared with published mass spectrometry data (PXD030044, PXD023676). E Immunoprecipitation of LDHA was used to detect its interaction with endogenous BAP1. F Immunoprecipitation of exogenous BAP1 was used to detect its interaction with LDHA. G Simultaneous knockdown of LDHA in the BAP1 knockdown and control groups, respectively, and detection of changes in extracellular lactate levels. *p < 0.05, **<0.01, ***<0.001, and no significance (ns).

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